Journal: Brain research bulletin

Article Title: The effects and mechanisms of AM1241 in alleviating cerebral ischemia-reperfusion injury.

doi: 10.1016/j.brainresbull.2024.111025

Figure Lengend Snippet: Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs (ERK, JNK, P38). n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.

Article Snippet: Primary antibodies against p-p38 (28796–1-AP, Proteintech), p38 (14064–1-AP, Proteintech), p-ERK (28733–1-AP, Proteintech), ERK (11257–1-AP, Proteintech), p-JNK (80024–1-RR, Proteintech), JNK (66210–1-Ig, Proteintech), IL-6 (ab233706, Abcam), TNF-α (ab183218, Abcam), NOX2 (ab310337, Abcam), BAX (50599–2- Ig, Proteintech), Cleaved-Caspase3 (C-Caspase3) (25128–1-AP, Proteintech) were added and incubated at 4◦C overnight.

Techniques: Protein-Protein interactions, Activity Assay, Western Blot, Phospho-proteomics

Journal: Journal of Chemical Ecology

Article Title: Structure-Function Analysis of Volatile ( Z )-3-Fatty Alcohols in Tomato

doi: 10.1007/s10886-025-01557-7

Figure Lengend Snippet: pH and MAPK effects of FAlcs compared to logP numbers and sensitivity to fusicoccin

Article Snippet: 20 μg of protein (15-well gels; Bio-Rad Mini-PROTEAN tetra system) was analyzed for MAPK phosphorylation by SDS PAGE followed by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H + L)-HRP conjugate; Bio-Rad) at a 1:20,000 dilution, followed by a chemiluminescence assay with the ClarityTM Western ECL substrate (Bio-Rad).

Techniques: Concentration Assay

Journal: Journal of Chemical Ecology

Article Title: Structure-Function Analysis of Volatile ( Z )-3-Fatty Alcohols in Tomato

doi: 10.1007/s10886-025-01557-7

Figure Lengend Snippet: Comparison of MAPK phosphorylation in response to ( Z )-3-FAlcs. ( A ) SP cells were treated for 5–45 min with 5 mM ( Z )-3-butenol, -hexenol, -heptenol, and -octenol (Z3-4-OL to Z3-9-OL) that were solved in 10 µL ethanol, and with 10 µL ethanol (Eth) alone. Phosphorylation of MAPKs was visualized by immunoblotting using an antibody against the phosphorylated MAPK activation motif pTEpY. Coomassie-stained membranes are shown to demonstrate equal loading and transfer. One of three independent experiments is shown (for all experiments, see Supp. Figure ). ( B ) MAPK phosphorylation signals in response to the five ( Z )-3-FAlcs were compared by experiment based on quantification of MPK1/2 and MPK3 signals as explained in Methods and Materials. ( C ) MAPK phosphorylation induced by 1 to 5 mM ( Z )-3-octenol and ( Z )-3-nonenol. SP cells were treated for 5 and 10 min with either 5 mM, 2.5 mM, or 1 mM of ( Z )-3-octenol or ( Z )-3-nonenol, or with 10 µL ethanol. Phosphorylation of MAPKs was determined as described under (A). One experiment and the corresponding coomassie-stained membrane is shown. Results of all three independent experiments are shown as described under (B). All experiments are shown in Supp. Figure

Article Snippet: 20 μg of protein (15-well gels; Bio-Rad Mini-PROTEAN tetra system) was analyzed for MAPK phosphorylation by SDS PAGE followed by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H + L)-HRP conjugate; Bio-Rad) at a 1:20,000 dilution, followed by a chemiluminescence assay with the ClarityTM Western ECL substrate (Bio-Rad).

Techniques: Comparison, Phospho-proteomics, Western Blot, Activation Assay, Staining, Membrane